Peptic–tryptic Digest of Gliadin Differentiates Human Monocytes into Dendritic Cells Independently of the Presence of Celiac Disease and Genetic Make-up
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The researchers matured dendritic cells taken from venous blood of patients with both active and with treated celiac disease, along with DQ2–DQ8-positive or negative control subjects. They treated the Dendritic cells with a peptic–tryptic digest of gliadin (500 μg/ml)
and assessed activation by means of fluorescent-activated cell sorting analysis, cytokine secretion, and the cells' ability to trigger T cell proliferation.
The team noted that gliadin up-regulated interleukin (IL)-6, IL-8, and IL-12 (p40) secretion in dendritic cells and triggered clear expression of the maturation markers human leukocyte antigen (HLA)-DR, CD25, CD83, and CD86 in all test subjects, without regard to their Genotype or the presence of disease; whereas the digest of bovine serum albumin had no effect.
However, gliadin-stimulated dendritic cells from patients with active celiac disease showed greater stimulation of autologous T cells compared to the other groups. The team concluded that further research should be aimed at identifying the mechanisms that control inflammation in healthy individuals.
J Clin Immunol, Volume 29, Number 1, January, 2009
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