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      Frequently Asked Questions About Celiac Disease   04/24/2018

      This Celiac.com FAQ on celiac disease will guide you to all of the basic information you will need to know about the disease, its diagnosis, testing methods, a gluten-free diet, etc.   Subscribe to Celiac.com's FREE weekly eNewsletter   What is Celiac Disease and the Gluten-Free Diet? What are the major symptoms of celiac disease? Celiac Disease Symptoms What testing is available for celiac disease?  Celiac Disease Screening Interpretation of Celiac Disease Blood Test Results Can I be tested even though I am eating gluten free? How long must gluten be taken for the serological tests to be meaningful? The Gluten-Free Diet 101 - A Beginner's Guide to Going Gluten-Free Is celiac inherited? Should my children be tested? Ten Facts About Celiac Disease Genetic Testing Is there a link between celiac and other autoimmune diseases? Celiac Disease Research: Associated Diseases and Disorders Is there a list of gluten foods to avoid? Unsafe Gluten-Free Food List (Unsafe Ingredients) Is there a list of gluten free foods? Safe Gluten-Free Food List (Safe Ingredients) Gluten-Free Alcoholic Beverages Distilled Spirits (Grain Alcohols) and Vinegar: Are they Gluten-Free? Where does gluten hide? Additional Things to Beware of to Maintain a 100% Gluten-Free Diet What if my doctor won't listen to me? An Open Letter to Skeptical Health Care Practitioners Gluten-Free recipes: Gluten-Free Recipes
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    Microwave Treatments of Wheat Can Confuse R5-antibody ELISA Gluten Tests


    Jefferson Adams


    • Microwave treatments of wheat can trick R5-antibody ELISA tests to misread actual gluten content.


    Celiac.com 02/17/2017 - In recent tests, researchers found that microwave treatment (MWT) of wet wheat kernels caused a striking reduction in R5-antibody-based ELISA gluten readings, reducing the readings to under 20 ppm, so that wheat could theoretically be labeled as gluten-free. However, the actual gluten content of the wheat remained unchanged. Just the test reading changed.


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    The research team included C Gianfrani, G Mamone, B la Gatta, A Camarca, L Di Stasio, F Maurano, S Picascia, V Capozzi, G Perna, G Picariello, A Di Luccia. They are variously affiliated with the Institute of Protein Biochemistry, CNR, Naples, Italy, the Institute of Food Sciences, CNR Avellino, Italy, the Department of the Sciences of Agriculture, Food and Environment at the University of Foggia, Italy, the Institute of Food Sciences, CNR Avellino, Italy; Department of Agriculture, University of Naples, Portici (Na), Italy, the Department of Clinical and Experimental Medicine, University of Foggia, Foggia (Italy) and National Institute of Nuclear Physics, Section of Bari, Italy, and the Department of the Sciences of Agriculture, Food and Environment, University of Foggia, Italy.

    The failure of R5 Elisa to register gluten in MWT stands in stark contrast to analysis of gluten peptides by G12 antibody-based ELISA, mass spectrometry-based proteomics, and in vitro assay with T cells of celiac subjects, all three of which gave consistent results both before and after MWT.

    As to what caused the R5 Elisa to misread the MWT samples, an SDS-PAGE analysis and Raman spectroscopy showed that MWT reduced the alcohol solubility of gliadins, and altered the access of R5-antibody to the gluten epitopes. Thus, MWT neither destroys gluten nor modifies chemically the toxic epitopes, this contradicts claims that MWT of wheat kernels detoxifies gluten.

    This study provides evidence that R5-antibody ELISA alone is not effective to determine gluten levels in thermally treated wheat products.

    Gluten epitopes in processed wheat should be monitored using strategies based on combined immunoassays with T cells from celiacs, G12-antibody ELISA after proteolysis and proper molecular characterization.

    Source:


    Image Caption: Photo: CC--Marcin Wichary
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    Guest coloradosue

    Posted

    Question 1: are companies that produce gluten free products using the R5 Elisa test alone to verify that their products are gluten free? Even in this country, there is no guarantee that some unscrupulous manufacturer would use bare minimum testing standards to produce a product for consumption while gaining maximum profit at our expense. Question 2: with cost cutting practices being used in all aspects of the health care industry, food/non-food industries, how do people know what tests are being used to verify the presence of celiac Disease (and other non-celiac diseases) when they truly do not know what's going on with their health? Even with information so readily available from so many sources, we, the patient and consumer, are vulnerable to exposure to gluten contamination which could have detrimental and devastating effects no matter how careful we are! With each visit to the emergency room because of gluten contamination, vigilance on my part becomes paramount to living the best way I know how. It would be nice to know that measures for testing were also paramount as well.

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    Guest Amanda Y

    Posted

    Important information to know! Would these microwave waves be the equivalent of at home microwaves or something more industrial? Separate issue: Has anyone else noticed issues with microwave food in general? (For example, there are times I make a safe meal at home and do not get sick, but if I microwave the same gluten free meal at work for lunch the next day, I get ill--similar to gluten, though a bit different. I know microwaves work fast by breaking the cell walls of food, but I'm not sure why I would feel ill due to that.)

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    Article: Microwave treatments of wheat can trick R5-antibody ELISA tests to misread actual gluten content. I was wondering what the government was going to do with the unsaleable wheat that is rotting in the silos. Celiac is a wide-ranging disease. Intake of gluten, yeast or yeast extract, egg, dairy, seed or flower oils results in dumping. Only cold pressed oils do not contain toxins. I told a co-worker; "If you want to know what foods and additives are toxic to the body, let me know, I'll ingest it and get right back to you!"

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    Jefferson Adams
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    Source:
    PLoS One. 2011;6(9):e24455. doi: 10.1371/journal.pone.0024455. http://www.ncbi.nlm.nih.gov/pubmed/20948997

    Jefferson Adams
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    Source:
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    Jefferson Adams
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    Sources:
    ncbi.nlm.nih.gov  Celiac.com ncbi.nlm.nih.gov  mendfamily.com