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    Eliminating the Celiac Disease-triggering Properties of Alpha-Gliadin Peptides in Wheat


    Jefferson Adams

    Celiac.com 02/16/2011 - A team of researchers recently set out to establish a universal approach to eliminate disease-triggering properties of alpha-gliadin peptides in celiac disease.


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    The research team included Cristina Mitea, Elma M. J. Salentijn, Peter van Veelen, Svetlana V. Goryunova, Ingrid M. van der Meer, Hetty C. van den Broeck, Jorge R. Mujico, Veronica Monserrat, Luud J. W. J. Gilissen, Jan Wouter Drijfhout, Liesbeth Dekking, Frits Koning, and Marinus J. M. Smulders.

    They are affiliated with the Department of Immunohematology and Blood Transfusion at Leiden University Medical Center, in Leiden, Plant Research International at Wageningen UR, and the Allergy Centre Wageningen, in Wageningen, The Netherlands.

    Celiac disease is triggered by an uncontrolled immune response to gluten, a mix of wheat storage proteins that include α-gliadins. Research has shown that α-gliadins contain several major epitopes involved in celiac disease pathogenesis.

    Eliminating such epitopes from α-gliadins would be a major step towards eliminating gluten toxicity for celiac disease patients.

    The team analyzed over 3,000 expressed α-gliadin sequences from 11 types of bread wheat to figure out if they encode for peptides that might play a role in celiac disease.

    The team synthesized all epitope variants they identified as peptides. They then tested each to see if it bound to the disease-associated HLA-DQ2 and HLA-DQ8 molecules, and if it was recognized by patient-derived α-gliadin specific T cell clones.

    For each of the α-gliadin derived peptides involved in celiac disease, the team found several specific naturally occurring amino acid substitutions that eliminate the celiac disease-triggering properties of the epitope variants.

    Finally, the team proved at the peptide level that by using systematic introduction of such naturally occurring variations α-gliadins, they can generate genes that no longer encode antigenic peptides. That is, they can create genes in wheat that do not trigger celiac disease.

    Their work offers an important contribution for developing strategies to modify gluten genes in wheat so that it becomes safe for people with celiac disease and gluten intolerance.

    The findings of the study also provide information for design and introduction of safe gluten genes in other cereals, which would conceivably make them both better in quality, and safe for people with celiac disease.


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    I wonder if this new grain will be accepted though since it will be a genetically modified organism (GMO).

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    Guest betsy frahm

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    Keep on trying. I am so grateful that you are doing this research and can't wait till you solve the problem and I can feel happy eating a good old sandwich again!

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    Sources:
    1. Toft M, Dietrichs E. Aggravated stuttering following subthalamic deep brain stimulation in Parkinson’s disease--two cases. BMC Neurol. 2011 Apr 8;11:44.
    2. Tani T, Sakai Y. Stuttering after right cerebellar infarction: a case study. J Fluency Disord. 2010 Jun;35(2):141-5. Epub 2010 Mar 15.
    3. Lundgren K, Helm-Estabrooks N, Klein R. Stuttering Following Acquired Brain Damage: A Review of the Literature. J Neurolinguistics. 2010 Sep 1;23(5):447-454.
    4. Jäncke L, Hänggi J, Steinmetz H. Morphological brain differences between adult stutterers and non-stutterers. BMC Neurol. 2004 Dec 10;4(1):23.
    5. Kell CA, Neumann K, von Kriegstein K, Posenenske C, von Gudenberg AW, Euler H, Giraud AL. How the brain repairs stuttering. Brain. 2009 Oct;132(Pt 10):2747-60. Epub 2009 Aug 26.
    6. Galantucci S, Tartaglia MC, Wilson SM, Henry ML, Filippi M, Agosta F, Dronkers NF, Henry RG, Ogar JM, Miller BL, Gorno-Tempini ML. White matter damage in primary progressive aphasias: a diffusion tensor tractography study. Brain. 2011 Jun 11.
    7. Lundgren K, Helm-Estabrooks N, Klein R. Stuttering Following Acquired Brain Damage: A Review of the Literature. J Neurolinguistics. 2010 Sep 1;23(5):447-454.
    8. [No authors listed] Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 43-1988. A 52-year-old man with persistent watery diarrhea and aphasia. N Engl J Med. 1988 Oct 27;319(17):1139-48
    9. Molteni N, Bardella MT, Baldassarri AR, Bianchi PA. Celiac disease associated with epilepsy and intracranial calcifications: report of two patients. Am J Gastroenterol. 1988 Sep;83(9):992-4.
    10. http://ezinearticles.com/?Food-Allergy-and-Stuttering-Link&id=1235725 
    11. http://www.craig.copperleife.com/health/stuttering_allergies.htm 
    12. https://www.celiac.com/forums/topic/73362-any-help-is-appreciated/
    13. Ford RP. The gluten syndrome: a neurological disease. Med Hypotheses. 2009 Sep;73(3):438-40. Epub 2009 Apr 29.
    14. Hadjivassiliou M, Gibson A, Davies-Jones GA, Lobo AJ, Stephenson TJ, Milford-Ward A. Does cryptic gluten sensitivity play a part in neurological illness? Lancet. 1996 Feb 10;347(8998):369-71.

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