Frederik Willem Janssen is head of the Chemistry Department, Food Inspection Service in Zutphen, and a subsidiary of the Inspector of Health Protection (similar to the FDA in America). Their lab has a special interest in.... modified gluten, edible packaging materials (which may contain gluten), and detection of hidden gluten in foods, including the development of improved detection methods. He is also a member of the Medical/Scientific Advisory Committee of the Dutch Celiac Society.
I found a report in a periodical by the Flemish Celiac Society of an investigation that was published in 1992 about immunological determination of gluten in beer and some distilled liquor. This confirmed our findings that the gluten content of beer is quite variable (the authors found levels from zero to 400 mg /liter gluten).
They did find gluten in distilled liquor! The levels varied from zero to 200-mg gluten/liter. The highest amount was found in a "Creme de Framboise" (200 mg/liter) but second was a French brandy VSOP with a score of 180 m g/liter. A Dutch gin was negative, which might be an indication that gluten in these type of liquor is not a carry over to the distillate! My guess is that this gluten is derived from the caramel coloring, though there is no proof about this yet. I always advise sensitive patients to abstain from brown colored liquor!
I would like to stress that the determination of gluten in these types of products is very unreliable and we have to count with false positive as well as false negative values. The gluten proteins could have been broken down to small (but still toxic) peptides and in that case a sandwich-type ELISA might produce false negative results because in that case you always need to two epitopes (binding sites for the antiserum) on one molecule to get a positive reaction. A competitive type assay would be the choice for this type of product, but we havent tried this type of analysis yet. We did use it on a soy sauce, which was prepared with wheat gluten and didnt find any gliadin, which might be an indication that gluten had been broken down to very small peptides with less than one binding site.
Frederik Willem Janssen, Zutpen, The Netherlands.
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