No popular authors found.


Get Celiac.com's E-Newsletter

Categories

No categories found.







Ads by Google:


Questions? Join Our Forum:
~1 Million Posts
& Over 66,000 Members!



SHARE THIS PAGE:
Celiac.com Sponsors:

Is Celiac Disease Linked to Rotavirus Protein? By Roy Jamron

Celiac.com 09/29/2006 - A new study identified a peptide which causes an immune reaction in a majority of active celiac disease patients but no such reaction in any celiac disease patients on a gluten-free diet. Antibodies to this celiac peptide also recognize and bind to the rotavirus protein VP-7 and cause increased intestinal permeability. Antibodies to VP-7 produced in rabbits also increase intestinal permeability. The celiac peptide also binds to Toll-like receptor 4 and activates monocytes (white blood cells active in innate immunity.) IgA and IgG antibodies to rotavirus protein VP-7 are present in a majority of celiac disease patients and to a much lesser percent of the general population. This suggests VP-7 may be involved in the pathogenesis of celiac disease through a molecular mimicry mechanism.

Below is the abstract of the study:

PLoS Medicine Volume 3, Issue 9, SEPTEMBER 2006
In Celiac Disease, a Subset of Autoantibodies against Transglutaminase Binds Toll-Like Receptor 4 and Induces Activation of Monocytes

Ads by Google:

Methods and Findings:
"In our attempt to clarify the pathogenesis of celiac disease, we screened a random peptide library with pooled sera of patients affected by active disease after a pre-screening with the sera of the same patients on a gluten-free diet. We identified a peptide recognized by serum immunoglobulins of patients with active disease, but not by those of patients on a gluten-free diet. This peptide shares homology with the rotavirus major neutralizing protein VP-7 and with the self-antigens tissue transglutaminase, human heat shock protein 60, desmoglein 1, and Toll-like receptor 4. We show that antibodies against the peptide affinity-purified from the sera of patients with active disease recognize the viral product and self-antigens in ELISA and Western blot. These antibodies were able to induce increased epithelial cell permeability evaluated by transepithelial flux of [3H] mannitol in the T84 human intestinal epithelial cell line. Finally, the purified antibodies induced monocyte activation upon binding Toll-like receptor 4, evaluated both by surface expression of activation markers and by production of pro-inflammatory cytokines."

Conclusions:
"Our findings show that in active celiac disease, a subset of anti-transglutaminase IgA antibodies recognize the viral protein VP-7, suggesting a possible involvement of rotavirus infection in the pathogenesis of the disease, through a mechanism of molecular mimicry. Moreover, such antibodies recognize self-antigens and are functionally active, able to increase intestinal permeability and induce monocyte activation. We therefore provide evidence for the involvement of innate immunity in the pathogenesis of celiac disease through a previously unknown mechanism of engagement of Toll-like receptor 4."

Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



Comments




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


I'm hoping to donate to celiac research this year. Does anyone have recommendations as to where to donate? Celiac Disease Foundation? Celiac research at a hospital or university? Thanks!

Thank you so much for your responses!! It is so hard because there are so many unknowns, inconclusive results, and lots of waiting!! I didn't realize it was so difficult to even get the tests to begin with. Thank you both SO much. I feel much better & am more comfortable going into my doctors app...

Just looking at its name, one might wonder if buckwheat is safe for people on a gluten-free diet. However, unlike its name, buckwheat does not naturally contain any wheat or gluten. As a result, buckwheat is generally safe for people with celiac disease on a gluten-free diet. Turns out that buc...

Oh my I just re read your post. Ok now I am going to definitely say yes-you need to find someone who will test you. If this Dr. will not you may want to look for one who will. I slowly reveal my issues on here and I still keep things close to the vest. My pregnancies/labor/deliveries we...

I try not to take a lot of ibuprofen as I know it's not great for my gut. But, when I need it, I take Costco's Kirkland ibuprofen. It is labelled gluten free and I have had no issues with it.