Celiac Disease and Gluten-Free Diet Support
- Questions? Join our forum: Nearly 1 MILLION POSTS, and over 62,000 MEMBERS!
Follow / Share
|Get Email Alerts|
- Safe Gluten-Free Food List (Safe Ingredients)
- Unsafe Gluten-Free Food List (Unsafe Ingredients)
- Gluten-Free Alcoholic Beverages
- Celiac Disease Symptoms
- The Gluten-Free Diet 101 - A Beginner's Guide to Going Gluten-Free
- Interpretation of Celiac Disease Blood Test Results
- Is Buckwheat Flour Really Gluten-Free?
Prolyl Endoprotease Enzyme May Allow Patients with Celiac Disease to Safely Eat Gluten on Occasion
Enzyme Quickly Breaks Down Wheat Protein
Celiac.com 05/23/2007 - The results of a study recently published in the journal Gut suggest that the enzyme prolyl endoprotease from Aspergillus niger (AN-PEP) taken along with meals might allow patients with celiac disease to safely consume gluten on occasion.
The negative effects of celiac disease are due in large part to an immune response to gluten.
A team of doctors in the Netherlands set out to assess the abilities of a post-proline cutting enzyme, prolyl endoprotease from Aspergillus niger (AN-PEP) in breaking down gluten. The research team was made up of doctors Cristina Mitea (1), Robert Havenaar (2), Jan Wouter Drijfhout (1), Luppo Edens (3), Liesbeth Dekking (1)* and Frits Koning (1).
The study was not performed on actual celiac patients, but used a dynamic system that mimics the human gastrointestinal tract (TIM system). Using the TIM system, the team performed two experiments. The first used the TIM-system to process a slice of bread with and without the presence of AN-PEP. The second experiment used the TIM-system to process standard fast food items, again both with and without the presence of AN-PEP.
Samples of the digesting food were taken from the TIM systems stomach, duodenum, jejunum and ileum compartments from zero to four hours after the beginning of the experiment. These samples were evaluated for levels of immunogenic peptides from gliadins and glutenins by monoclonal antibody based competition assays, Western blot analysis and proliferation T-cell assays.
Results of both experiments showed that AN-PEP broke down gluten in the stomach so effectively that almost no gluten reached the duodenum compartment. Because these results show that AN-PEP is capable of speeding the breakdown of gluten in a gastrointestinal system that closely mimics live digestion, the team concluded that AN-PEP might offer celiac patients an opportunity to stray from their strict gluten free diets from time to time.
1 Dept of Immunohematology and Blood Transfusion,
Leiden University Medical Center, Leiden, Netherlands.
2 TNO Quality of Life, Zeist, Netherlands
3 DSM Food Specialties, Delft, Netherlands
Gut. Published Online First: 9 May 2007. doi:10.1136/gut.2006.111609health writer who lives in San Francisco and is a frequent author of articles for Celiac.com.
Celiac.com welcomes your comments below (registration is NOT required).
Histological, Serological and Symptomatic Responses to Gluten Challenge in Adults with Celiac Disease
A diagnosis of Celiac disease is measured mainly by an adverse response to gluten, yet there is very little in the way of data regarding gluten challenge in adults on a gluten-free diet.... [READ MORE]
A Systematic Review of Diagnostic Testing for Celiac Disease Among Patients With Abdominal Symptoms
Clinical presentation of celiac disease can vary considerably from patient to patient.... [READ MORE]
Future Enzyme Treatment Possible for People with Celiac Disease
J Pharmacol Exp Ther.... [READ MORE]
Duodenal Histology in Patients with Celiac Disease after Treatment with a Gluten-free Diet
This new study
emphasizes the importance of following a strict gluten-free diet, and
getting regular follow-up biopsies after your diagnosis.... [READ MORE]
Jefferson Adams is a freelance writer living in San Francisco. His poems, essays and photographs have appeared in Antioch Review, Blue Mesa Review, CALIBAN, Hayden's Ferry Review, Huffington Post, the Mississippi Review, and Slate among others.
He is a member of both the National Writers Union, the International Federation of Journalists, and covers San Francisco Health News for Examiner.com.View all articles by Jefferson Adams
In Celiac.com's Forum Now:
Celiac.com Celiac Disease & Gluten-Free Diet Forum - All Activity