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Isolation and Culture of Fibroblasts from Endoscopic Duodenal Biopsies of Celiac Patients

Celiac.com 06/23/2009 - Fibroblasts are one of the most important components in inflammation and tissue remodeling process, and are thought to be involved in the pathogenesis of autoimmune disorders such as celiac disease.

Attempts to better understand the role played by fibroblasts in celiac diseases have been hampered by the absence of specific models.

A team of researchers recently set out to isolate and culture primary fibroblasts from endoscopic duodenal biopsies of celiac and non-celiac subjects, and to analyze their growth patterns and any morphometric characteristics.

The research team obtained 60 tissue samples via duodenal biopsy from 20 celiac patients and 114 samples from 38 non-celiac subjects. The team then mechanically chopped and enzymatically digested in order to obtain primary cell cultures.

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Using cultured cells, the researchers charted growth patterns, karyotype (Q-banding analysis), expression of typing proteins (fibroblast surface protein and cytokeratin 20) and morphometric parameters (diameters and their ratio, perimeter, area and perimeter/area ratio at computerised image analysis)

They were able to successfully culture primary cells in 78% of the collected duodenal biopsies. Cultured cells, expressing the fibroblast surface protein, tested negative for cytokeratine 20 and maintained a normal kariotype. Cells grew slowly with no differences between the celiac and the non-celiac group. Morphometric analysis of celiac fibroblasts showed substantially increased size, with a preserved diameters ratio, and a reduced perimeter/area ratio.

For the first time, researchers have shown the feasibility of culturing primary fibroblast cells from endoscopic duodenal biopsies in celiac and non-celiac subjects.

This ability shows promise for enhancing studies intended to establish the role of fibroblasts as a possible partaker in the pathogenesis of the celiac mucosal damage.


Source: Journal of Translational Medicine 2009, 7:40

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REALLY odd call out here, I am attending a anime convention called A-Fest in Dallas come August, I need someone to split the room with it who is gluten-free. I take extra precautions, I COOK all the food, bring only CERTIFIED foods into the room, The room will be Gluten Free, Corn Free, Dairy Free, Peanut Free. I am trying to find someone to split the room cost with, that would be safe to be around I CAN NOT AFFORD to get sick at one of these things, it is one of my few joys left in life and get very paranoid around them. So I need someone who is also gluten-free to make sure the room stays safe (YES I have done with with a non celiac with the rules down and well stuff happens so not chancing it). Room split is food coverage comes to $400 if it is just two people. 4 day convention, I will arrange a meal plan around your diet as long as it is free of my allergens. I will also provide various snacks, baked goods, and even stuff to take home with you. https://animefest.org/ ^Convention info.

Hi Jennifer, This thread might have some information that would help you. Your doctors are pretty lame IMHO. Perhaps you can find a celiac group in your area that has local meetings for support. They might also suggest a different doctor who knows how to treat celiac patients.

All the above posts are full of good advice. What I'd like to add is, if you have coeliac disease and continue to eat gluten, you run the risk of other autoimmune diseases in the future as well as osteoporosis, malnutrition and even cancer, so even if you had no symptoms at the beginning, and may also not have any symptoms if you eat gluten (not all coeliacs do), the damage is still being done to your gut and the rest of your body, so please be aware of this.

You could possibly try calling the places in Texas and Chicago to see if they can refer you somewhere that does accept your insurance. Oh good luck to you!

Hi Jennifer and welcome CyclingLady has given you some good advice above. You want certainty and that's entirely understandable. Go back to your doctors and explain that you need to know a little more and hopefully they will engage positively with you. If they don't, then do pursue a second opinion. I just wanted to address your last paragraph quoted above. The problem with celiac, or in my case non celiac gluten sensitivity, is that it presents or doesn't present in so many different ways. It can do hidden damage which may take many years to become apparent. It can impact in ways which are incredibly difficult to recognise or isolate. I am 'lucky' in that the way that gluten impacts on me is far worse than any mental or social isolation brought upon by the diet, so motivation is easy for me, even without the certainty of a celiac diagnosis, there really is no alternative, I don't think I'd last long on a gluten diet now. But I can well understand how difficult it may be to stay honest on the diet if you don't have any symptoms to deal with. The diet can be isolating, there does become a distance between you and 'normal' people. Who would want to deal with all that if they didn't have to? If you aren't satisfied with your doctors responses and choose to go back onto gluten I suggest you find another doctor and go back into the diagnostic process and properly exclude celiac, including a scope. Otherwise you could be taking a big risk with yr long term health. You may find that this process supplies you with an answer as if your diagnosis was correct your response to the reintroduction of gluten may surprise you, or not of course! best of luck!