No popular authors found.


Get Celiac.com's E-Newsletter

Categories

No categories found.







Ads by Google:


Questions? Join Our Forum:
~1 Million Posts
& Over 66,000 Members!



SHARE THIS PAGE:
Celiac.com Sponsors:

Polymeric Binders Block Gliadin-induced Intestinal Cell Toxicity

Celiac.com 10/16/2009 - A team of researchers recently set out to investigate the ability of a polymeric binder to reverse the toxic effects induced by gliadin in human intestinal cells and gliadin-sensitive HCD4-DQ8 mice. The team was made up of Maud Pinier, Elena F. Verdu, Mohamad Nasser–Eddine, Chella S. David, Anne Vézina, Nathalie Rivard, and Jean–Christophe Leroux.

The team neutralized gliadin through complexation to a linear copolymer of hydroxyethylmethacrylate (HEMA) and sodium 4-styrene sulfonate (SS). They then examined the ability of the polymeric binder to mitigate the damaging effect of gliadin on cell-cell contact in IEC-6, Caco-2/15, and primary cultured differentiated enterocytes.

They used gliadin-sensitive HLA-HCD4/DQ8 transgenic mice to measure the effectiveness of the polymeric binder in averting gliadin-triggered intestinal barrier dysfunction.  They found that Poly(hydroxyethylmethacrylate-co-styrene sulfonate) [P(HEMA-co-SS)] complexed with gliadin in a fairly precise manner.

Ads by Google:

Exposing intestinal cells to gliadin caused major changes in both cell structure and cell to cell contacts. By complexing the gliadin with P(HEMA-co-SS) the researchers were able to prevent these undesirable changes. More importantly, the P(HEMA-co-SS) inhibited gliadin digestion by gastrointestinal enzymes, which minimized the development of peptides that trigger immune adverse immune reactions.

By co-administering P(HEMA-co-SS) with gliadin to HLA-HCD4/DQ8 mice, researchers were able to eliminate gliadin-triggered changes in the gut barrier and lower intraepithelial lymphocyte and macrophage cell counts.

From these results, the team concludes that polymeric binders can prevent in vitro gliadin-induced epithelial toxicity and intestinal barrier dysfunction in HCD4/DQ8 mice. Such polymeric binders might play a significant role in the treating people with gluten-induced disorders.

Source:
GASTROENTEROLOGY 2009;136:288–298

Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



2 Responses:

 
Hallie
Rating: ratingfullratingfullratingfullratingemptyratingempty Unrated
said this on
16 Oct 2009 4:19:45 PM PST
Wouldn't an experiment in mice like this one be considered "in vivo," rather than, "in vitro?"

 
Maggie
Rating: ratingfullratingfullratingfullratingfullratingfull Unrated
said this on
31 Jan 2010 12:54:17 PM PST
Very interesting article. Sign me up if they would like a live person who is gluten intolerant to test this on.




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


Yep. Initially I had the full panel. DGP was the only positive and it's the only one my doctor orders now.

Well I wish mine was dia. earlier, I got all kinds of other food issues, and other auto immune disease that came up as complications. If you deal with and change over now you can prevent a even more limited diet. I was running a bucket list thinking I was going to die before my dia. I had slight ...

So, do they just test your DGP like they just test my TTG?

The only test I have had done is the TTG because that's what I had done initially after taking matters into my own hands and going to my local health fair. Celiac is so common they do that screening at our health fair. My number was so high that my doctor didn't order other labs and went straight...

Celiac used to be considered a children's disease - so, by the old standards, you are a bit old to be diagnosed. We now know that Celiac can start at any age. I don't know if you are a male or a female, but untreated Celiac can lead to miscarriages and infertility. And all the other stuff...