No popular authors found.
Ads by Google:

Categories

No categories found.


Get Celiac.com's E-Newsletter





Ads by Google:


Follow / Share


  FOLLOW US:
Twitter Facebook Google Plus Pinterest RSS Podcast Email  Get Email Alerts
SHARE:

Popular Articles

No popular articles found.
Celiac.com Sponsors:

Characterization of IL-17A-Producing Cells in Celiac Disease Mucosa

Celiac.com 02/03/2010 - Celiac disease increases production of IL-17A by cells that also make IFN-gamma. Recently, a research team set out to characterize the expression of IL-17A-producing cells in celiac disease.

The team included I. Monteleone, M. Sarra, G. Del Vecchio Blanco, O. A. Paoluzi, E. Franzè, D. Fina, A. Fabrizi, T. T. Macdonald, F. Pallone, and G. Monteleone of the Department of Internal Medicine at the University of Tor Vergata in Rome, Italy.

Infiltration of the mucosa with IFN-gamma-secreting Th1 cells is one of the features associated with celiac disease. Recent studies have shown the pathogenic effects previously attributed to Th1 cells may in fact be caused by a novel subset of T cells, termed Th17 cells, and noted for expressing high levels of IL-17A.

In this study, the team set out to characterize the expression of IL-17A-producing cells in celiac disease. Using real-time PCR and ELISA, the team showed that expression of IL-17A RNA and protein is greater in active celiac disease biopsy specimens than in specimens from inactive celiac disease, and normal mucosal biopsies.

Through flow cytometry, the team confirmed that the mucosa of celiac disease patients overproduces IL-17A, and that the main sources for this overproduction were CD4(+) and CD4(+)CD8(+) cells.  Most IL-17A-producing CD4(+) and CD4(+)CD8(+) cells co-expressed IFN-gamma but did not co-express CD161.

Ads by Google:

Including a peptic-tryptic digest of gliadin to ex-vivo organ cultures of duodenal biopsy specimens taken from patients with inactive celiac disease enhanced IL-17A production 
by both CD4(+) and CD4(+)CD8(+) cells.

Since the team showed earlier that patients with celiac disease overproduced IL-21, a T cell-derived cytokine involved in the control of Th17 cell responses, they next determined whether IL-21 was responsible for regulating IL-17A expression.

Blocking IL-21 action with a neutralizing IL-21 Ab lowered total IL-17A expression in cultures of active celiac disease and peptic-tryptic digest of gliadin-treated celiac disease biopsy specimens.

From the data, the team concludes that celiac disease increases IL-17A, which is produced by cells that also produce IFN-gamma.

Source: Journal of Immunology, 2010 Jan 8.

Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



Comments




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


Once your tTg is in the normal range, and it's only 1 point away from that, that would be considered excellent results. tTg just has to be in the normal range to be called a success. The number you want to be as low as possible within the normal range is the DGP or AGA testing, as that tests fo...

I started a topic a couple of weeks ago about my type 1 daughter with a ttg iga result of greater than 100 being suspected of having celiac. She saw the GI and has her biopsy scheduled for this coming Tuesday. Seems like there is no doubt that it will show celiac but we will know soon. I have ...

Hi all, my TTG/iga came back. It was 4. 0-3 is considered negative and 4-10 is weak positive. In January it was 12 and before my endoscopy in October it was >100. it plummeted those first 2 months but seems to be taking more time to get lower. Should I feel optimistic? I guess 4 s...

Yes, i was 230 lbs upon diagnosis. My crp had been high for over 10 yrs. The first year i lost 70 lbs of water. Then i started to realize my continued irratic gi problems were more intollernces from processed foods as well as things like dairy and legumes. I went on an elimination diet and lost 2...

Thanks for the well wishes Jmg. It appears that you & cycling lady think the sugar maybe our issue, let me clarify that's not our issue for it is manifesting as one of our additive intolerance exposure symptoms. One both my son and I have discovered over 2016. It's not a sugar crash type or a gi ...