No popular authors found.


Get Celiac.com's E-Newsletter

Categories

No categories found.







Ads by Google:


Questions? Join Our Forum:
~1 Million Posts
& Over 66,000 Members!



SHARE THIS PAGE:
Celiac.com Sponsors:

Can Vitamin C Reduce Mucosal Immune Inflammatory Response to Gliadin in Celiac Disease Patients?

Celiac.com 03/28/2012 - A clinical research team wanted to determine if adding ascorbate (vitamin C) to gliadin-stimulated biopsy culture could reduce the mucosal immune response to gliadin in people with celiac disease.

Photo: CC - articotropical The research team included D. Bernardo, B. Martínez-Abad, S. Vallejo-Diez, E. Montalvillo, V. Benito, B. Anta, L. Fernández-Salazar, A. Blanco-Quirós, J. A. Garrote, and E. Arranz. They are affiliated with the Mucosal Immunology Lab of the Department of Paediatrics & Immunology at Spain's Universidad de Valladolid-CSIC.

Their quest was fueled by the understanding that the IL-15/NF-κB axis plays a key role in celiac disease. Because ascorbate is known to inhibit effects on NF-κB, the IL-15/NFκB axis looks like a good possible molecular target for reducing gliadin-induced inflammation in celiac disease.

For their study, the team conducted in vitro gliadin challenges (100 μg/ml) on duodenal biopsy explants from treated patients with celiac disease. Challenges were conducted with and without 20mM ascorbate. As an internal control, the team used an extra tissue explant in basal culture.

The team then measured secretion levels of nitrites (3h), and IFNγ, TNFα, IFNα, IL-17, IL-13, and IL-6 (24h) on the supernatants. They measured IL-15 using western-blot on whole protein duodenal explants.

Ads by Google:

When the team added ascorbate to in vitro culture gliadin-challenged biopsies, they found that the ascorbate blocked secretion of nitrites (p=0.013), IFNγ (p=0.0207), TNFα (p=0.0099), IFNα (p=0.0375), and IL-6 (p=0.0036), as compared with samples from culture that received no ascorbate.

They also found that the addition of ascorbate reduced cytokine secretion to levels even lower than those observed in basal cultures (IFNγ: p=0.0312; TNFα: p=0.0312; IFNα: p=0.0312; and IL-6: p=0.0078).

Moreover, the gliadin-challenge triggered IL-15 production in biopsies from treated celiac disease patients, while IL-15 was completely blocked in the cultures that received ascorbate.

Interestingly, ascorbate completely blocked IL-15 production even in the only treated celiac disease-patient who showed basal IL-15 production.

From these results, the team concludes that ascorbate reduces the mucosal inflammatory response to gluten in an in vitro biopsy culture. As such, ascorbate might offer supplementary benefits in future celiac disease therapy.

Source:

Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



1 Response:

 
vivienne harris
Rating: ratingfullratingfullratingfullratingfullratingempty Unrated
said this on
02 Apr 2012 8:09:45 PM PST
I guess I will start taking a little of that.




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


I didn't mean my world fell apart because of celiac but because of something else. Finding out about celiac disease actually gave me hope.

@PosterboyOne thing is though I never get heartburn. And this only happens when I've consumed something for consecutive days, like I had the bananas and too many nuts. But if PH has something to do with it then maybe when I'm eating more vegetables and broth that's why I usually feel much better,...

I have seen this a few times....run a gluten free bakery....one of my previous customers has the disease, but he gawks at my prices and then goes eats from other stores and consumes gluten products.....I tried offering freebies left over items to get him to switch....been watching him degrade ove...

I don't know, it's probably best described as a lot of bile, sometimes yellow, sometimes green (sorry) and a lot of mucus (sorry_) and when I eventually throw it up (if it gets to that point) it's burning in my throat, so I equate it with acid. Also because of the term "antacid" I think of it as...

I'm a scientist to be (currently a student..) And i know how you feel