No popular authors found.


Get Celiac.com's E-Newsletter

Categories

No categories found.







Ads by Google:


Questions? Join Our Forum:
~1 Million Posts
& Over 66,000 Members!



SHARE THIS PAGE:
Celiac.com Sponsors:

Researchers Try to Identify Origins of Abnormal Cells in Refractory Celiac Disease Type II

Celiac.com 09/21/2012 - Refractory celiac disease type II (RCDII) is a severe complication of celiac disease that occurs when symptoms and intestinal damage continue even when the patient adopt a gluten-free diet. Refractory celiac disease marked by abnormal intraepithelial lymphocytes (IELs) of unknown origin that display an atypical CD3(-)CD7(+)icCD3(+) phenotype. About 40% of patients with RCDII lymphocytes develop a dangerous and invasive lymphoma.

Photo: CC--RDECOMA team of researchers recently sought to identify possible origins of abnormal intraepithelial lymphocytes in refractory celiac disease type II.

The research team included F. Schmitz; T.M. Tjon, Y. Lai; A. Thompson; Y. Kooy-Winkelaar; R.J. Lemmers; H.W. Verspaget; M.L. Mearin; F.J. Staal; M.W. Schreurs; T. Cupedo; A.W. Langerak; C.J. Mulder; J. van Bergen; and F. Koning.

In their study, the researches sought to find the physiological counterpart of these abnormal intraepithelial lymphocytes cells.

To do so, they used microarray analysis, real-time quantitative PCR and flow cytometry to compare RCDII cell lines with T-cell receptor positive (TCR(+)) IEL (T-IEL) lines. They then used their data to identify cells with an RCDII-associated phenotype in duodenal biopsies from non-refractory individuals by multicolor flow cytometry.

They found that RCDII cell lines were distinct from T-IEL lines and showed higher levels of multiple natural killer (NK) cell receptors.

Ads by Google:

In addition to the CD3(-)CD7(+)icCD3(+) phenotype, the RCDII lines showed an absence of CD56, CD127 and CD34, compared with other lymphocyte subsets.

Moreover, they found cells matching this surface lineage-negative (Lin(-)) CD7(+)CD127(-)CD34(-) phenotype that showed a functional interleukin-15 (IL-15) receptor and comprised a substantial portion of IELs in duodenal specimens of patients without celiac disease, particularly children. They also found cells of this kind in the thymus.

For patients without celiac disease, the Lin(-)CD7(+)CD127(-)CD34(-) subset was one of four subsets within the CD3(-)CD7(+)icCD3(+) population that showed a differential expression of CD56 and/or CD127.

The results indicate that the CD3(-)CD7(+)icCD3(+) population is heterogeneous and show the existence of a Lin(-) subset that is different from T, B, NK and lymphoid tissue inducer cells.

The team hypothesizes that the IL-15 cells are the counterpart of abnormal cells that are expanded in RCDII and transformed in RCDII-associated lymphoma.

Source:

Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



Comments




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


i am thinking you may be LADA, latent autoimmune diabetes of adult, type one, of course. Not to hit you when you are down, but now is the time to realize that you eat to live, not live to eat. find a different source of sensual enjoyment, ie photo, painting, singing, etc. and "hold your nose"...

You might consider the endoscopy. Like I said, some celiacs (about 10%) have negative blood tests. The endoscopy can rule out other issues too (like Crohn?s). Not all celiacs are wasting away either. I hope they figure it out and you feel better fast!

Yes, I do not take iodine salt as well (forgot to add it), I get bubbles after it... That actually worries me a little because in long run what is my thyroid going to say (I do not eat seafood so there is no source of iodine for me at all)? I tried taking iodine supplement but got bad reaction to...

I was eating wheat and gluten at the time of the test. No test or diagnoses was done while I was in hospital they just Gave me a list of could be?s and nothing was done they just sent me home once the sepsis was gone. I went to my auto immune dr because they thought I had lupus or hypothyroid and...