No popular authors found.


Get Celiac.com's E-Newsletter

Categories

No categories found.







Ads by Google:


Questions? Join Our Forum:
~1 Million Posts
& Over 66,000 Members!



SHARE THIS PAGE:
Celiac.com Sponsors:

T-bet and pSTAT-1: New Genetic Markers for Celiac Disease?

Celiac.com 01/18/2013 - Up-regulation of T-bet and phosphorylated signal transducers and activators of transcription (pSTAT)1 are key transcription factors for the development of T helper type 1 (Th1) cells, and have been found in the mucosa of patients with untreated celiac disease.

CC--Mervi EskelaninA team of researchers recently set out to determine if T-bet and pSTAT-1 expression in PBMC from celiac disease patients might offer new genetic markers of disease activity.

The research team included G. Frisullo, V. Nociti, R. Iorio, A.K. Patanella, D. Plantone, A. Bianco, A. Marti, G. Cammarota, P.A. Tonali, A.P. Batocchi. They are affiliated with the Department of Neurosciences at Catholic University in Rome, Italy.

For their study, the team used transcription factor analysis to determine whether T-bet and pSTAT1 expressions are up-regulated in the peripheral blood of celiac disease patients, and if they correlate with disease activity.

They used flow cytometry to analyse T-bet, pSTAT1 and pSTAT3 expression in CD4(+), CD8(+) T cells, CD19(+) B cells and monocytes from peripheral blood of 15 untreated and 15 treated celiac disease patients and 30 controls. They also conducted a longitudinal study of five celiac patients before and after treatment with a gluten-free diet.

For their evaluation, the team used enzyme-linked immunosorbent assay (ELISA), interferon (FN)-gamma, interleukin (IL)-17 and IL-10 production by peripheral blood mononuclear cell (PBMC) cultures.

Ads by Google:

They found that T-bet expression in CD4(+), CD8(+) T cells, CD19(+) B cells and monocytes and IFN-gamma production by PBMC was higher in untreated than in treated celiac disease patients and control subjects.

They also found that pSTAT1 expression was higher in CD4(+)T cells, B cells and monocytes from untreated celiac disease patients than from treated patients and control subjects.

Compared with treated celiac disease patients and control subjects, untreated patients showed increased pSTAT3 only in monocytes.

They confirmed their results using data obtained from the longitudinal evaluation of transcription factors.

From their results, they conclude that flow cytometric analysis of pSTAT1 and T-bet protein expression in peripheral blood mononuclear cells could be useful and sensible markers in the follow-up of celiac disease patients to evaluate disease activity and response to dietary treatment.

Source:

Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



Comments




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


I wish I had been diagnosed at 19. In retrospect, I was having symptoms as a 16 year old, and didn't get diagnosed until I was almost 30. That delay created a lot of havoc, and other problems that are now permanent (an aggressive case of rheumatoid arthritis and violent reactions to contamination...

From the Chicago Celiac Disease Center which is one of the premier celiac disease research & treatment centers in the world: Are you scheduled for a biopsy? Are you eating gluten? Any changes in your diet can affect the accuracy of your biopsy result...

My panel was the same as yours........all tests positive by large numbers so you can consider yourself a Celiac. Are you very symptomatic? I will add that I did not eat Whole Foods exclusively when healing. I needed to gain weight badly so ate gluten-free bread and a few other things that ...

My allergist did suggest I cut out gluten right away, but I?ll call the GI tomorrow and discuss that. Thank you!

He only did 2 of the full panel & you are positive on one of them, the DGP IgG. It only takes one positive to move on to an endoscopy. Here's the full celiac serum panel: Anti-Gliadin (AGA) IgA Anti-Gliadin (AGA) IgG Anti-Endomysial (EMA) IgA Anti-Tissue Transglutaminase (tTG) Ig...