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Autophagy Protein LC3 Tied to Accumulation of Dendritic Cells in Celiac Disease

Celiac.com 04/28/2014 - Accumulation of dendritic cells (DCs) in duodenal mucosa is associated with celiac disease. Autophagy protein LC3 has recently been implicated in autoantigen formation. However, its role in celiac disease remains unknown.

Image: Wikimedia Commons.A team of researchers recently set out to examine role of autophagic protein LC3 expressed by activated DCs in celiac disease. The research team included P. Rajaguru, K. Vaiphei, B. Saikia, and R. Kochhar, with the Department of Histopathology, Post Graduate Institute of Medical Education and Research in Chandigarh, India.

The team analyzed thirty celiac disease patients at initial presentation and after 6 months of gluten-free diet (GFD). They examined duodenal biopsies for histological changes and CD11c, CD86, and MAP1LC3A expressions by double immunohistochemistry (IHC).

They used Masson's trichrome (MT) staining to determine basement membrane (BM) thickness and Oil Red O (ORO) staining for mucosal lipid deposit. They also conducted polymerase chain reaction (PCR) for the HLA-DQ system. For statistical analysis, they used paired and unpaired t test, chi-square test, Fisher's exact test, and McNemar-Bowker test. A P-value

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They found HLA-DQ2 and HLA-DQ8 alleles in all study subjects. They also observed thicker BM in 63% of subjects, while 73% of subjects showed ORO-positive lipid in surface lining epithelium.

Pre-treatment biopsies showed a higher number DCs expressing LC3, which dropped significantly in follow-up biopsies, and also showed significant reduction in BM thickness and ORO.

These results show that histological improvement in duodenal biopsies is connected with less activated DCs expressing autophagic protein, which likely play important role in the development of celiac disease and other autoimmune disorders.

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I have been having issues with gluten for quite some time now and decided to go gluten free back in October, I finally got the celiac blood test a couple weeks ago and it came back inconclusive. My doctor referred me to a gastroenterologist who I saw today, she told me I'd have to eat gluten rich foods for 1 month and then have another blood test done....of it comes back negative she said I'd need to eat gluten another few weeks (up to another month) and then do a scope and biopsy....this would be hell on me as I have severe cramping and nausea when I am exposed to gluten. I haven't actually eaten anything that is straight gluten like bread so I'm not sure what would happen. She gave me the option to hold off and continue my diet or go through 2 months of pure gluten exposure (and pain) my question is has anyone been faced with this? Should I just continue with my gluten free diet and assume I have it or is the diagnosis really that important? Doc says based on my symptoms and reactions I more than likely have it.

Hello everyone, My name is Alessio, I'm a 25 year old male and for 24 of my 25 years I've been healthy as a rock. Things changed in July 2016 when I decided to quit smoking using Chantix. 3 weeks in the treatment to quit smoking I started suffering from insane anxiety (an entire week of depersonalization) and daily panic attacks, I was hospitalized for a week and sent home with Clonazepam (Klonopin) for the following couple of months which at the time helped a lot. At the time I was quite convinced that what I went through must have been a side effect of chantix but almost a year later and with the Celiac diagnosis I sort of suspect that quitting smoking might have triggered Celiac Disease for me, hence the panic attacks and the anxiety. Around November 2016 I started suffering from throat flares, which was found to be acid reflux ( I very rarely suffered from heartburn, maybe a couple of occasions) but the throat flare was constant, from the moment I ingested something in the morning till when I went to bed. Turned out to be caused by Celiac as well (as soon as i started my gluten-free diet in late December the flare just vanished over a week time). I had my biopsy 5 days ago, on the 20th of April and in the GI notes he wrote that there were no signs of cancer, acid damage or ulcers but he could see villous atrophy. Leading up to the biopsy I had to go back to eating gluten for a month a half and that's where the current problems started. I have had a sore throat for the last 4 and a half weeks and 2 lymph nodes very close to the adam's apple (just above it, slightly to the side on both sides) have gotten hard. I've had an ultrasound on the one on my left ( which was the very first one to appear) and it showed a 2cm growth that showed no sign of malignancy, this node at the time was tender and moveable ( a good sign I've been told). On the right side of my neck the node seems to be hard and not moveable. I've had a CT scan for my neck ( I haven't personally seen the results yet) but my doctor does not seem concerned. I have been tested for Strep and Mono and both turned out to be negative. 2 weeks ago I started suffering from a very acute burning sensation just between my breasts and shoulders on both sides, it lasted for 3 days straight and woke me up several times and the fourth day i woke up with soreness ( which still persists) in the same areas that were burning, this lead my doctor to test me for Rheumatoid Factor and presence of ANA, the RF turned out to be 10 ( <12 is not indicative of disease) and my ANA were positive to a titer of 1:80 with a speckled pattern, again my doctor shows no signs of concern regarding this. I am suffering from chronic fatigue at the moment and being a carpenter that really doesn't make life easy. I have lost around 20 pounds in the last 2 months, went from my standard 180 pounds to 160 and to today I don't know if I can attribute that to my gluten-free diet ( and minor cutoff of junk food) or whether there might be a second underlying issue going on right now. All in all since doing my gluten challenge I haven't been feeling well. My question is, can what's been going on in the last month ( hard nodes, sore throat, fatigue, weight loss and inflammation outside my gut) be just another reaction to gluten? I'm 5 days into the gluten-free diet again and no improvements yet, I know that it usually takes a couple of weeks to have effect. Thanks for your time, and I have learned tons from this website about dealing with Celiac Disease, Thank you. Alessio

@cyclinglady thanks for checking in Restricted diet didn't do much. Still had some VA last time they checked. Heath still otherwise fine, so RCD remains unlikely. My sxs kick in lockstep with life stress, so that kind of points to some general IBS stuff on top of celiac disease. Very doubtful I'm getting any gluten in, but fingers crossed my system is just a little hyper-vigilant, as I ponder on this thread.

I have always noticed that the table wine in Europe is pretty damn good! I am a wine lover and so is my husband but he does like his Green's beer.

The reason they set the limit at 20ppms is that through scientific study, they have proven that the vast majority of people with Celiac Disease do not have an autoimmune reaction to amounts below that......it is a safe limit for most. Also, just because that limit is set at 20ppms, does not mean that gluten-free products contain that amount of gluten. Testing for lower levels becomes more expensive with each increment down closer to 0-5ppms, which translates into higher priced products. Unless you eat a lot of processed gluten-free food, which can have a cumulative affect for some, most people do well with the 20ppm limit.