No popular authors found.
Ads by Google:

Categories

No categories found.


Get Celiac.com's E-Newsletter





Ads by Google:


Follow / Share


  FOLLOW US:
Twitter Facebook Google Plus Pinterest RSS Podcast Email  Get Email Alerts
SHARE:

Popular Articles

No popular articles found.
Celiac.com Sponsors:

New Human Anti-tTG type IgA Test Kit is Highly Sensitive and Specific for the Detection of Celiac Disease

Celiac.com 06/25/2003 - Below is an abstract of yet another study that supports the use of human anti-tTG type IgA serological tests to accurately diagnose celiac disease:

Alimentary Pharmacology & Therapeutics
Volume 17 Issue 11 Page 1415 - June 2003
Antibodies to human recombinant tissue transglutaminase may detect coeliac disease patients undiagnosed by endomysial antibodies
N. Tesei*, E. Sugai*, H. Vázquez*, E. Smecuol*, S. Niveloni*, R. Mazure*, M. L. Moreno*, J. C. Gomez, E. Mauriño* & J. C. Bai*

Background: The screening and diagnosis of coeliac disease have been simplified by the advent of new serological tools.

Ads by Google:

Aim: To assess the clinical utility of a newly developed kit for antibodies to human recombinant tissue transglutaminase (hu-anti-tTG) in a large population of patients undergoing intestinal biopsy for suspected intestinal disorders.

Methods: We evaluated 426 serum samples from consecutive adult patients (250 from untreated coeliac disease patients and 176 from individuals in whom a diagnosis of coeliac disease had been excluded), obtained at the time of intestinal biopsy. Samples were tested for immunoglobulin A (IgA) hu-anti-tTG by enzyme-linked immunoabsorbent assay, IgA endomysial antibodies (EmA) by indirect immunofluorescence and IgA and IgG antigliadin antibodies by enzyme-linked immunoabsorbent assay. A sub-group of samples was also assessed for a guinea-pig-based anti-tissue transglutaminase.

Results: According to the cut-off for hu-anti-tTG, the sensitivity, specificity and positive and negative predictive values were 91%, 96%, 97% and 87%, respectively. Simultaneous determination of EmA showed values of 86%, 100%, 100% and 83% for the same parameters. Although 19 coeliac disease patients (7.6%) were negative for EmA and hu-anti-tTG, both tests rendered superior statistical values to antigliadin antibody tests. At diagnosis, IgA deficiency was detected in 11 patients, but both assays were able to detect samples with mild to moderate deficiency. The comparison of hu-anti-tTG with EmA showed excellent concordance between the tests ( statistic, 0.85). Discordance was observed in 20 samples from coeliac disease patients (8%) and in nine samples from controls (5%). Fifteen samples had an EmA-negative but hu-anti-tTG-positive serology, and five showed the converse pattern. Comparison of human recombinant and guinea-pig tests showed concordant results in 96% of cases.

Conclusions: The quantitative determination of hu-anti-tTG type IgA using a commercial enzyme-linked immunoabsorbent assay kit was highly sensitive and specific for the detection of coeliac disease. Our results in a large population of patients with a clinical condition suggestive of the disorder demonstrated that the test can be used to detect a substantial number of patients otherwise unrecognized by IgA EmA.

Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



Comments




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


Thankyou both of you for your advice, i will have the endoscopy will just have to wait, my blood tests were not hitting the roof with reading but a low to medium result, dont want to be one of them people who have gone gluten free with no need, however i do show typical symptons of celiac but no ...

You are normal! All the things you are going through are normal for someone with celiac disease....probably with any life changing illness. What you need is patience. Most here take a year to heal or longer due to setbacks on the diet or healing from systemic damage. The nervous system seems ...

Yes! Ask for the IgA deficiency test! Why? Well, your doctor did not even follow protocol. In order to validate the IgA celiac tests, he should have determined if you are IgA deficient or not. http://www.mayomedicallaboratories.com/it-mmfiles/Celiac_Disease_Diagnostic_Testing_Algorithm.p...

Almost EVERY single celiac expert in the world and/or groups like the U.K./U.S. Gastroenterologist Associations recommend obtaining biopsies for diagnosing celiac disease. There are some exceptions. If you are too ill to even risk an endoscopy, your celiac panel is extremely high on several of ...

No, I didn't get a endoscopy or even a blood test. I got digestive as well as other problems though on a number of occasions after being gluten free and accidentally eating something with gluten. My symptoms were severe and dramatic so it was obvious to me that I had celaic disease. I wou...