No popular authors found.


Get Celiac.com's E-Newsletter

Categories

No categories found.







Ads by Google:


Questions? Join Our Forum:
~1 Million Posts
& Over 66,000 Members!



SHARE THIS PAGE:
Celiac.com Sponsors:

German Researchers Develop New Ultra-Sensitive Test for Gluten in Foods

Nahrung. 2003 Oct;47(5):345-8.

Ads by Google:

Celiac.com 01/14/04 – German researchers have developed a new test to determine the level of gliadin, the portion of gluten that is toxic to celiac patients, in foods. This new technique is called immunopolymerase chain reaction (iPCR), and it utilizes immunological detection of gliadin by a monoclonal antibody R5 conjugated when an oligonucleotide is amplified by PCR. The technique yields a "30-fold above the level reached by enzyme immunoassay" in laboratory tests, and it detects concentrations in food "as low as 0.16 ng/ml corresponding to 16 microgram gliadin/100 g food or 0.16 ppm (corresponding to 0.25 g of food extracted in 10 ml of solvent and 25-fold dilution of the extract prior to analysis)." This is the first time that this highly sensitive technique has been used for gliadin analysis, and "is the first approach to perform real-time iPCR in one step without changing the reaction vessels after enzyme immunoassay for subsequent PCR analysis thus minimizing risks of contamination and loss of sensitivity."

Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



Comments




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


I'm probably the most annoying person on this forum. Lately I've been posting everyday? ok wondering a few brand options for products just need a list.... Pickles: Green olives: Banana peppers: Lunchmeat: turkey/salami/pepperoni Hard/block cheese (cheddar or colby): (was...

Hi Your tests indicates that you are positive for Celiac. I have a problem with the diagnose too. I have recently started to research about gluten intolerance and I have so many symptoms... It's like all the health issues I had all my life have an answer now. I think my mother had ...

Yeah mine was the constipation really bad, it clears up some but my constipation was partially trigger by the damage causing malabsoprtion of magnesium leading to the constipation along with other things and complications. All sore layers up into a big compounding issue, example the tiredness is ...

For me, the DH rash was what lead my Dr. to have me tested for celiac. Since I have IBS, I was thinking that was what was causing my stomach issues- and I was so bloated that I looked pregnant. It is still my DH that lets me know, I have somehow encountered gluten/cross contamination. My skin ...

Hi, this is my first post. I am wondering how to proceed. I tried going off gluten because I was diagnosed with Morphea and I read it might help. To my surprise, it cleared up a bunch of other symptoms big time. Then I read about celiac and found out I should not have stopped eating gluten if I w...