I also read this which some recommended as well: NASPGHAN recommends eating roughly 2 servings of gluten, equivalent to 2 slices of wheat-based bread, daily for 6-8 weeks prior to testing.

My dr. stated to eat gluten for 4 weeks before bloodtest. I feel so much better w/o gluten and was going to wait until after the summer vacation but hesitating since reading the antibodies might go away etc. and if I can get it over with before hand especially before daughters graduation next month and before summer might be nice. Any tips besides eating at least 4 slices of whole wheat to get 10g of gluten in for testing. Im more worried about when it hits to find a bathroom especially b/c it can be after eating a meal or might not hit until after 2 days etc. Should I just go back to eating normal to prepare or does it make sense eat 2 slices whole wheat 2x a day and the rest gluten free to prep for test? I noticed b4 gluten free diet I started 5 weeks ago I would have non gluten-free oatmeal and have to go to bathroom right after eating or 2 hrs later. In afternoon I would eat whole wheat and not need to go immediately to bathroom after eating. What steps did you take for test prep.

as the title says, just need to know that. i tried calling the support line but it's closed atm. i'm tired, in pain, and don't wanna set back recov. thx.

Most doctors don't even order anything besides the tTG-IGA and maybe total IGA when doing blood testing for celiac disease. The EMA is the very first celiac blood antibody test that was developed and has largely been replaced by the tTG-IGA which is less expensive to run in the lab. My understanding is they both pretty much check for the same thing. The IGG tests are second tier tools that are less specific for celiac disease. It is not at all uncommon for the EMA and the tTG-IGA to disagree with one another. We frequently see this on the forum. I do not know why. Please realize that doctors typically run a number of tests when diagnosing a medical condition. If there was one test that was foolproof, there would not be a need for other tests and other testing modalities. It works that way with many or most diseases. 

But yes seeing all those negatives does give me a bit of doubt! I feel confident but those results give me a seed of doubt. Especially since it's a lifelong diet for him!