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Incubation starts tomorrow


dixonpete

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I now have all my supplies and my microscope. I ended up buying a better one than I had planned because after doing extensive reading I decided I wanted a dissecting scope, and I justified the extra money by considering it a lifetime investment since I'll be using it for probably the next 20 or so years.

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Incubation will begin tomorrow and harvesting will occur 10 days after that. I'm not due for inoculation til April 5th, but there would be little harm in applying 5-10 collected larvae now to see how they fare.

From the Helminthic Therapy Wiki there are a wide variety of techniques that people employ for incubation and even the most practiced appear to experience failures. I have two months to get it right. Once I do succeed, I'll report back with my methods and materials.

I almost forget to mention that all is well gutwise. I'm 1/2 way through my 5-month hookworm cycle and my little symbionts appear to be doing their jobs well. I'm not experiencing any colitis or celiac symptoms whatsoever.

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dixonpete

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For those interested in a sneak peek of what I'm up to, the technique I'm trying is this one: https://www.facebook.com/groups/678894952216125/permalink/5400971020008471/

It's from the Helminthic Therapy Group on Facebook. You'll have to request to join the group to be able to see the post. No biggie, it's free, and if you have any intention on pursuing HT you'll want to join anyway.

If that's too foreboding, in a nutshell incubation is all about putting infected poop in an airtight jar with non-distilled, chlorine-free water, and then after 10 days or so very carefully using a microscope to fish out the L3 larvae from the water. Apparently it's harder than it sounds.

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