Pete Dixon

The incubation worked.

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The biggest expense was the microscope I bought off Amazon, an AmScope SE306. I picked it because it would give plenty of room for pipette work above the sample. I paid ~$370 Can.

The other supplies needed were variously sized pipettes, petri dishes, mineral water, adhesive gauze bandages, bleach for cleanup, paper towels, a garbage bag to cover my desk, and glasses for eye protection. And of course, most importantly, nitrile disposable gloves. For the incubation itself, I used a hermetically sealable glass jar from the dollar store. AliExpress was the source for the pipettes, petri dishes and bandages. The drug store for the gloves. Total cost for these ancillary items was under a $100.

Less than a teaspoon of stool was diluted and smeared at the top of a coffee filter that was suspended in the jar by a couple of threads. The larvae head down to the water (1/4" or less) at the bottom of the jar.

I didn't use much of the water from the jar. As a rough estimate, the total yield was probably 300+ L3 larvae. Incubation time was 12 days.

It took a couple of hours to get used to handling working with pipettes under the microscope. The biggest trick I learned was to only use a small drop on the petri dish not much larger than the viewing area of the scope. That made navigation much easier.  

I've seen quite complicated instructions online for incubating hookworms, with temperature controlled incubators and vermiculite. Turned out none of that was necessary.

If anyone has a mind of trying this, I'd just like to emphasize the need for care. Yes, the idea here is to get infected with hookworms, but only in a controlled manner. A careless splash might mean getting a 100 hookworm dose when you only needed 10. With hookworms, just like in medication, overdoses are a very bad thing!