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Question:  Do I have to re-introduce gluten in order to have an accurate gluten sensitivity test done?
 Answer: Yes and No
 
 If a person knows they are sensitive to gluten and have gone on a gluten-free diet, and want to know if they can have gluten again, then a  challenge is in order (reintroduce gluten). THIS IS STRONGLY NOT RECOMMENDED. The gluten challenge has many cases of people who were damaged (some permanently) from the reaction to reintroducing gluten Even Small Amounts of Gluten Cause Relapse in Children With Celiac Disease, Journal of Pediatric Gastroenterology and Nutrition 34:26­30,   and it is no longer a requirement for diagnosing celiac disease Am J Clin Nut 1999;69:354-65.
 
 Among 374 children in whom celiac disease was diagnosed before the age of 2, 5% developed an auto-immune disorder while on a gluten-free diet. Of those who went gluten-free, had years of no symptoms, then went back on a gluten-containing diet, 3.65% prevalence of systemic auto-immune disease with less than 12 months of eating gluten 9.1  % prevalence for 13-36 months of eating gluten again 26.3% prevalence for > 36 months of eating gluten again This means 1 out of 4 people who were sensitive to gluten, went gluten-free and eliminated all of their symptoms developed auto-immune diseases within 3 years of eating gluten again. Gastroenterology 1999;117:297-303.
 
 If you know you are Gluten Sensitive, and you've gone on a gluten-free diet, and you want to know "am I better", then testing will confirm you are being successful in 'quieting down' the inflammatory cascade that occurs with gluten sensitivity and which sets one up for the development of