Enterolab Results

[dlp252]

First, of all, thank you in advance for any help in understanding the results. I'm pretty clear on the fact that I have a gluten sensitivity and a casein sentivity but not sure I'm understanding what the genes mean and have a question about the fat issue. Here are the results:

Gluten Sensitivity Testing

Fecal Antigliadin IgA 15 Units (Normal Range <10 Units)

Fecal Antitissue Transglutaminase IgA 13 Units (Normal Range <10 Units)

Microscopic Fecal Fat Score: 67 Units (Normal Range < 300 Units)

HLA-DQ Gene Molecular analysis: HLA-DQB1*0302, 0301

Food Sensitivity Testing

Fecal anti-casein (cow's milk) IgA antibody 15 Units (Normal Range <10 Units)

Fecal anti-ovalbumin (chicken egg) IgA antibody 9 Units (Normal Range <10 Units)

Fecal Anti-Saccharomyces cerevisiae (dietary yeast) IgA 8 Units (Normal Range <10 Units)

***

HLA gene analysis reveals that you have a genotype containing the main gene

that predisposes to gluten sensitivity and celiac sprue: HLA-DQB1*0201

(HLA-DQ2) and/or HLA-DQB1*0302 (HLA-DQ8).

Interpretation Of HLA-DQ Testing by Molecular Analysis and Conversion to

"Serologic Equivalent": Today HLA-DQ gene testing is done by analyzing DNA

using molecular techniques. In the past, the methods were done by analyzing

blood cells for the antigens produced by the genes, and these past methods

were responsible for the most commonly known nomenclature for HLA-DQ genes

even today (using integers such as "DQ2"). These are called "serologic

equivalents" to the specifically analyzed gene material. The serologic

equivalents are as follows:

* If the first two numbers of the molecular type are 05, the serologic

equivalent is DQ1 subtype DQ5

* If the first two numbers of the molecular type are 06, the serologic

equivalent is DQ1 subtype DQ6

* If the molecular type is 0201, the serologic equivalent is DQ2 * If the

molecular type is 0301, the serologic equivalent is DQ3 subtype DQ7

* If the molecular type is 0302, the serologic equivalent is DQ3 subtype DQ8

* If the molecular type is 0303, the serologic equivalent is DQ3 subtype DQ9

* If the first two numbers of the molecular type are 03 but it is not 0301,

0302, or 0303, the serologic equivalent is DQ3

* If the first two numbers of the molecular type are 04, the serologic

equivalent is DQ4

The gluten sensitive, celiac genes are HLA-DQB1*0201 and HLA-DQB1*0302

(HLA-DQ2 and HLA-DQ8, respectively).

The other gluten sensitive genes are any molecular type involving another

HLA-DQB1*03 number (i.e., HLA-DQ3), or any HLA-DQB1*05 number, or any

HLA-DQB1*06 number (i.e., HLA-DQ1)

If you have one gluten sensitive gene, then your offspring have a 50%

chance of receiving the gene from you, and at least one of your parents

passed it to you. Having two copies of a gluten sensitive or celiac gene,

means that each of your parents, and all of your children (if you have

them) will possess at least one copy of the gene. Two copies also means

there is an even stronger predisposition to gluten sensitivity than having

one gene and the resultant immunologic gluten sensitivity or celiac disease

may be more severe.

Not sure I know exactly what this means other than it's possible I DO have celiac disease given my symptoms, but in any case I definitely have a sensitivity. Does all that mean I have one gene or two? Am I subtype 8 or 3...sorry, I don't have a scientific mind, so it all sounds like greek to me.

One other question. I don't seem to have problems with fat absorbtion, so that's good right...that means there is probably not a lot of damage??

Thanks in advance!!!!

[Matilda]

..

[dlp252]

You have DQ8. This is 0302. DQ2 and DQ8 are the ones most commonly associated with celiac disease.

You have DQ3, subtype 7. This is 0301. This is associated with gluten intolerance and maybe one of the rare types associated with celiac disease that are not 2 or 8. It is very similar to 8. There's something about it on the Enterolab somewhere.

I've got DQ2 plus this one.

Best wishes,

Matilda

Thank you so much! That helps!

[CMCM]

Just curious...which of the Enterolab tests did you get, and how long to get your results?

Also...I find it a bit disappointing that they would send out these results without a "layman's" translation of what it all means!

[dlp252]

Just curious...which of the Enterolab tests did you get, and how long to get your results?

Also...I find it a bit disappointing that they would send out these results without a "layman's" translation of what it all means!

I had the "Gluten Sensitivity Stool and Gene Panel Complete Plus Free Milk Sensitivity Stool Test" and I also ordered the yeast and egg panel. Those tests covered just about all of what I wanted tested...the only other thing I wish I had tested was soy. I sent the tests in the Wednesday after Thanksgiving (Nov 30), and received them on Monday, Dec. 12, so a little less than two weeks. The good news, I guess, is that I can eat eggs and yeast, lol.

Most of the results were fairly easily understood, I just can't wrap my mind around the gene thing. I did leave out some of the information...here's the full explanation:

Analysis of this stool sample indicates you have dietary gluten

sensitivity. For optimal health and prevention of small intestinal damage,

osteoporosis, damage to other tissues (like nerves, joints, pancreas, skin,

liver, among others), and malnutrition, recommend a strict gluten free

diet. If you are experiencing any symptoms, these may resolve following a

gluten free diet. As gluten sensitivity is a genetic syndrome, you may

want to have your relatives screened as well.

HLA gene analysis reveals that you have a genotype containing the main gene

that predisposes to gluten sensitivity and celiac sprue: HLA-DQB1*0201

(HLA-DQ2) and/or HLA-DQB1*0302 (HLA-DQ8).

Interpretation of Fecal IgA to Gliadin and Other Food Antigens: Levels of

fecal IgA antibody to a food antigen greater than or equal to 10 are

indicative of an immune reaction, and hence immunologic "sensitivity" to

that food. For any elevated fecal antibody level, it is recommended to

remove that food from your diet.

Values less than 10 indicate there currently is minimal or no reaction to

that food and hence, no evidence of food sensitivity to that specific food.

However, because 1 in 500 people cannot make IgA at all, and rarely, some

people can still have clinically significant reactions to a food antigen

despite the lack of a significant intestinal antibody reaction (because the

reactions primarily involve T cells), if you have an immune syndrome or

symptoms associated with food sensitivity, it is recommended that you try a

strict removal of suspect foods from your diet for 6-12 months despite a

negative test.

The numeric value of an antibody is not a measure of clinical

severity. Values of 10 Units can be associated with the same reactions as

the maximum values we measure (200-300). Most positive reactions are

between 20 and 80 Units. An analogy would be trying to use the level of

antibodies to penicillin in a person who has had an allergic reaction to

penicillin to determine if it would be safe for them to take penicillin

again. This obviously is not done because those with demonstrated

penicillin allergy could not take penicillin without the risk of suffering

severe health consequences. Although gluten sensitivity is not a true

allergy like penicillin allergy, the concept is the same.

Interpretation of Fecal IgA to the Human Enzyme Tissue Transglutaminase:

Values greater than or equal to 10 Units indicate that the immune reaction

to gliadin has resulted in an autoimmune reaction to the human enzyme

tissue transglutaminase. It is this autoantibody that may be responsible

for the many autoimmune diseases associated with gluten sensitivity.

Interpretation of Quantitative Fecal Fat Microscopy: A fecal fat score

less than 300 indicates there is no malabsorbed dietary fat in stool

indicating that digestion and absorption of nutrients is normal.

A fecal fat score greater than 300 Units indicates there is an increased

amount of dietary fat in the stool which usually is due to gluten-induced

small intestinal malabsorption/damage when associated with gluten

sensitivity. Values between 300-600 Units are mild elevations, 600-1000

Units moderate elevations, and values greater than 1000 Units are

severe. Any elevated fecal fat value should be rechecked in one year to

ensure that it does not persist because chronic fat malabsorption is

associated with osteoporosis among other nutritional deficiency syndromes.

Possible causes of elevated fecal fat scores besides gluten-induced damage

to the intestine include:

* Another inflammatory bowel disease (such as Crohn's disease which is

associated with gluten sensitivity)

* Deficiency in the production or secretion of pancreatic enzymes or bile salts

* Overgrowth of bacteria in the small intestine

* Diarrhea itself causing the fat to rush through the intestine unabsorbed

* Consuming very large amounts of dietary fat, eating unabsorbable

synthetic dietary fat substitutes or taking "fat blockers"

* Resection of the small intestine causing "short bowel syndrome"

Interpretation Of HLA-DQ Testing by Molecular Analysis and Conversion to

"Serologic Equivalent": Today HLA-DQ gene testing is done by analyzing DNA

using molecular techniques. In the past, the methods were done by analyzing

blood cells for the antigens produced by the genes, and these past methods

were responsible for the most commonly known nomenclature for HLA-DQ genes

even today (using integers such as "DQ2"). These are called "serologic

equivalents" to the specifically analyzed gene material. The serologic

equivalents are as follows:

* If the first two numbers of the molecular type are 05, the serologic

equivalent is DQ1 subtype DQ5

* If the first two numbers of the molecular type are 06, the serologic

equivalent is DQ1 subtype DQ6

* If the molecular type is 0201, the serologic equivalent is DQ2 * If the

molecular type is 0301, the serologic equivalent is DQ3 subtype DQ7

* If the molecular type is 0302, the serologic equivalent is DQ3 subtype DQ8

* If the molecular type is 0303, the serologic equivalent is DQ3 subtype DQ9

* If the first two numbers of the molecular type are 03 but it is not 0301,

0302, or 0303, the serologic equivalent is DQ3

* If the first two numbers of the molecular type are 04, the serologic

equivalent is DQ4

The gluten sensitive, celiac genes are HLA-DQB1*0201 and HLA-DQB1*0302

(HLA-DQ2 and HLA-DQ8, respectively).

The other gluten sensitive genes are any molecular type involving another

HLA-DQB1*03 number (i.e., HLA-DQ3), or any HLA-DQB1*05 number, or any

HLA-DQB1*06 number (i.e., HLA-DQ1)

If you have one gluten sensitive gene, then your offspring have a 50%

chance of receiving the gene from you, and at least one of your parents

passed it to you. Having two copies of a gluten sensitive or celiac gene,

means that each of your parents, and all of your children (if you have

them) will possess at least one copy of the gene. Two copies also means

there is an even stronger predisposition to gluten sensitivity than having

one gene and the resultant immunologic gluten sensitivity or celiac disease

may be more severe.

For more information about result interpretation, please see

Open Original Shared Link

Stool Analysis performed by: Frederick Ogunji, Ph.D., EnteroLab

Molecular Gene Analysis (if ordered) performed by: Laboratories at Bonfils

Interpretation of all results by: Kenneth D. Fine, M.D., EnteroLab

[minigimp]

Thanks Matilda

I have had a lot of trouble getting diagnosed because I didn't get a positive result from the standard test.

Naturally I am angry because I was consistently told I had IBS even though I had classic symptoms of Coeliac disease. After a biopsy, I recently developed severe pancreatic insufficiency, lichen sclerosis and blepharitis which wont go with antibiotics because of sjogrens. My typing is dqb1 0301 dq7. It is especially dangerous for anyone with this type to have an internal biopsy because tissue trauma will trigger off other immune disorders. This makes it diffucult to conclusively diagnose Coeliacs. This is also the type for MS and people with this disease often wind up with some form of paralysis after operations. It surprises me that people with MS are not usually encouraged to go on gluten free diets since gluten is a very likely cause. I have found out through the process of elimination that I also react to casein, and saccharomyces cerevisae. Casein dramatically effects the sclerosis. When I took SC as an antiviral I started having trouble breathing and haven't been right since. I wonder if anyone else on this site has my type, it's not that uncommon.

[confused]

You have DQ8. This is 0302. DQ2 and DQ8 are the ones most commonly associated with celiac disease.

You have DQ3, subtype 7. This is 0301. This is associated with gluten intolerance and maybe one of the rare types associated with celiac disease that are not 2 or 8. It is very similar to 8. There's something about it on the Enterolab somewhere.

I've got DQ2 plus this one.

Best wishes,

Matilda

Ok has anyone heard about this with 0301. ABout it might be one of the rare types associated with celiac.

paula

[nora-n]

I googled dq7 and celiac and right away got this:

Open Original Shared Link

"Eight of the 122 celiac disease patients lacked both the heterodimer and the DRB104 alleles; therefore, we further screened the DQB1 locus to investigate if other alleles were present in association to celiac disease (2)(14). Two of these eight celiac disease patients showed the DQ2 molecule (DQB10201 allele in heterozygosis); but one of them carried also the DQB10501 allele, also found in Sardinian patients to be associated to celiac disease (15); four showed the DQ7 molecule (DQB10304 or DQB10301 alleles). Another patient carried the DQ8 molecule encoded by the DQB10302 allele, and the remaining patient had the haplotype DQB10501 in homozygosis (15). The DQ8 molecule seems to be an alternative to DQ2 in influencing susceptibility towards celiac disease, being present in up to 20% of celiac patients not bearing of DQ2 in the Mediterranean area (2)(14)(16). Our data do not support an earlier finding that HLA-DQ7 is a nonsusceptible molecule (2). In fact, DQ7 was present in 50% of our celiac disease patients in the absence of the heterodimer and of the DRB104 alleles. Because the DQ7 molecule is very similar to the DQ8 molecule, it could alternatively present similar gluten-derived peptides to restricted T cells (17). DQ7 has been detected in a few celiac disease cases in the absence of DQ2 but in association with the DRB104 alleles (18).

The diagnostic power of HLA typing and of the immunological approach in celiac disease was calculated in 80 patients whose symptoms suggested celiac disease and is reported in TableUp 1B. At the celiac disease prevalence of 52% in our Center, the positive predictive value of the heterodimer was 88%, whereas the negative predictive value was 80%. EMAs were present in all celiac disease patients except two; one was 18 months old and had the heterodimer, the other presented the DQ7 molecule."