No popular authors found.
Ads by Google:

Categories

No categories found.


Get Celiac.com's E-Newsletter





Ads by Google:


Follow / Share


  FOLLOW US:
Twitter Facebook Google Plus Pinterest RSS Podcast Email  Get Email Alerts
SHARE:

Popular Articles

No popular articles found.
Celiac.com Sponsors:

New Blood Tests Can Spot Gluten Sensitivity Among Enteropathy Patients Who Test Negative for Anti–Tissue Transglutaminase


New Blood Tests for Celiac Disease

Celiac.com 04/20/2010 - A team of researchers recently set out to determine whether new serology assays can detect gluten sensitivity among enteropathy patients seronegative for anti–tissue transglutaminase.

Emilia Sugai, Hui Jer Hwang, Horacio Vázquez, Edgardo Smecuol, Sonia Niveloni, Roberto Mazure, Eduardo Mauriño, Pascale Aeschlimann, Walter Binder, Daniel Aeschlimann and Julio C. Bai comprised the research team.

They are variously affiliated with the Small Bowel Section of the Department of Medicine at C. Bonorino Udaondo Gastroenterology Hospital in Buenos Aires, Argentina, the Matrix Biology and Tissue Repair Research Unit at the Cardiff University School of Dentistry in Cardiff, UK, and with INOVA Diagnostics, Inc., of San Diego, California.

Some patients with celiac disease may not show a normal positive reaction to the test most commonly used for IgA anti–tissue transglutaminase (anti-tTG) antibodies.

The research team set out to determine the usefulness of newer assays incorporating synthetic deamidated gliadin-related peptides (DGPs), or other TG isoenzymes as antigen, for detecting gluten sensitivity in IgA anti-tTG–seronegative patients.

Ads by Google:

The team tested blood samples drawn at diagnosis from 12 anti-tTG–seronegative patients with a celiac-like enteropathy, from 26 patients with skin biopsy–proven dermatitis herpetiformis (DH) and, lastly, from 26 patients with IgA anti-tTG–positive celiac disease.

All patients showed typical levels of total IgA. On each patient, the team conducted intestinal biopsy and serum testing for detection of IgA and IgG isotypes of both anti-DGP and anti-tTG in a single assay (tTG/DGP Screen; INOVA Diagnostics). They also tested each patient for simultaneous detection of both IgA and IgG anti-DGP antibody isotypes (DGP Dual; INOVA Diagnostics). Lastly, they tested each patient for the detection of antibodies to transglutaminase 3 (TG3) or transglutaminase 6 (TG6).

All patients who showed positive anti-tTG results also tested positive in anti-DGP assays.

The tTG/DGP Screen caught six of the 19 anti-tTG seronegatives (31.6%), while anti-DGP Dual produced caught five of these cases (26.3%). Whereas both assays detected 2 anti-tTG–negative DH patients with partial villous atrophy, they were positive in only 2 of the 5 cases with no histologically discernible mucosal damage. Testing for antibodies to TG3 and TG6 caught seven of the 19 anti-tTG–negative patients (36.8%), five of whom also tested positive for anti-DGP.

From these results, the team concludes that using tTG/DGP Screen, or anti-DGP Dual, to detect anti-DGP improves diagnostic sensitivity of gluten sensitive patients with non–IgA- deficiency, or anti-tTG–seronegativity, and celiac-like enteropathy. The same enhancement is also achieved by detecting antibodies to other TG isoenzymes.

Source:


Celiac.com welcomes your comments below (registration is NOT required).












Related Articles



1 Response:

 
Waitinggame
Rating: ratingfullratingfullratingfullratingfullratingempty Unrated
said this on
04 May 2012 10:34:38 AM PDT
Interesting! I tested negative to tTG and total Iva was normal, yet I was five times above positive marker on GDP IgA test! Still infesting phase and waiting for biopsy to see if I have celiac. Thanks for posting this!




Rate this article and leave a comment:
Rating: * Poor Excellent
Your Name *: Email (private) *:




In Celiac.com's Forum Now:


There aren't any gluten free nut brand in my country. Are nuts with shells like peanuts,walnuts sold in stores without package gluten free? Do they add anything else to them?

Supplementation with Natural Vitality Stress & Energy, will help a lot it is a sublingual B-vitamin pretty much. I use it with the Neurological support from the same company and Natural Vitality Calm Magnesium. These along with a diet in foods that help with mood like snacking on cocoa nibs, hemp...

Hey everyone... newbie here. I have a few questions but first my history: Dx with Hashimoto's in 2003. Had SEVERE adverse reaction to T4 no idea why but then switched to T3. But T4 opened a can of worms. Immediately after starting T4 had digestive issues. Aften a few years tried gluten free....

My 7-year-old has been complaining of constant itchiness and a skin crawling sensation for the past five months, but has no external rash or symptoms. It seems to get worse at night, although that may be because it's more difficult to distract herself at bedtime. We've tried switching shampoo...

Wow! It sounds like you are going through a lot right now. Most of our dinners at home are gluten free and my kids eat the food just fine. It does take a bit more planning and you have to make more things from scratch. Our favorite dinners are tacos (serve on corn tortillas), red beans and...