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Cross Contamination

Jestgar

By Jestgar
in Introduce Yourself / Share Stuff

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Jestgar Rising Star
Jestgar
Posted
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kareng Grand Master
kareng
Posted

Excellant!

pricklypear1971 Community Regular
pricklypear1971
Posted

Now that was creative!

jerseyangel Proficient
jerseyangel
Posted

Loved it--the first time cross contamination hasn't pissed me off :P

Lisa Mentor
Lisa
Posted

pissed me off :P

Patti said "pissed me off" :lol::lol::lol: <3

Nice visual Jess!

lpellegr Collaborator
lpellegr
Posted

That was great! Between cell culture and celiac disease, cross-contamination takes up a good part of my thinking, but never quite so entertainingly. Thanks!

Jestgar Rising Star
Jestgar
Posted
  • Author

My favorite part is the music. :)

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Jestgar Rising Star
Jestgar
Posted
  • Author

I think this was the most bizarre entry in the contest:

Jestgar Rising Star
Jestgar
Posted
  • Author

And a Canadienne won a prize:

kareng Grand Master
kareng
Posted

And a Canadienne won a prize:

That was great! the other was odd.

Skylark Collaborator
Skylark
Posted

That was great! Between cell culture and celiac disease, cross-contamination takes up a good part of my thinking, but never quite so entertainingly. Thanks!

Hahaa! Cell culture is awfully good training for thinking about gluten. All people living with celiacs should have to carry a cell line antibiotic free for two months. Then they would "get" it! :lol:

Jestgar Rising Star
Jestgar
Posted
  • Author

Hahaa! Cell culture is awfully good training for thinking about gluten. All people living with celiacs should have to carry a cell line antibiotic free for two months. Then they would "get" it! :lol:

I haven't used antibiotics in my cultures for years. :rolleyes:

Skylark Collaborator
Skylark
Posted

I haven't used antibiotics in my cultures for years. :rolleyes:

Nice! I grew cells antibiotic-free in grad school but in industry we couldn't afford the higher risk of downtime so we used pen/strep. We did train beginners by having them carry a couple lines antibiotic-free in an incubator we didn't mind them contaminating.

Jestgar Rising Star
Jestgar
Posted
  • Author

I started in industry, but doing an assay that required antibiotic free, so I've never bothered adding them back. I bit of googling has convinced me that abx are inactivated after a couple of times in the water bath anyway, so it's more of a psychological security blanket than a real one.

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    • Zuma888
      Hypothetical question about antibodies

      By Zuma888 · Posted

      Thank you so much.
    • knitty kitty
      Hypothetical question about antibodies

      By knitty kitty · Posted

      You have one gene for Celiac.  You have a second autoimmune disease, Hashimoto's thyroiditis, which is frequently found at a higher rate with Celiac.  HLA genes carry autoimmune disease genes like Celiac and Hashimoto's and diabetes and others.   You have Celiac symptoms of reacting after gluten.  You said "I am however still suffering from the effects of the gluten challenge (food sensitivities, slight brain fog, weird stool, fatigue, swollen thyroid, bodyaches)."  And your anti-thyroid antibodies increase after gluten exposure.  While tTg IgA does not directly attack the thyroid, gluten exposure does trigger the  immune system to produce antibodies against the thyroid in genetically predisposed individuals.  You did not eat sufficient gluten (10 grams of gluten per day for two weeks minimum) to raise the autoimmune antibodies to the point they can be measured in the blood, so your blood tests may well be inaccurate.  You could choose to continue the gluten challenge of 10 grams a day for at least two weeks and get retested.   At the very least, you know that gluten is harmful to your thyroid, and because you are genetically predisposed to Celiac disease, a strict gluten free diet would be beneficial for your overall health.  
    • Zuma888
      Hypothetical question about antibodies

      By Zuma888 · Posted

      Thanks @Scott Adams! I guess my question now is: do the celiacs who can get away with regular contamination without villi damage as you mentioned have to be strict about cross-contamination ? 
    • Zuma888
      Hypothetical question about antibodies

      By Zuma888 · Posted

      Thank you very much @knitty kitty! I'm glad you brought up the point about histamine. I have been taking an antihistamine after meals where I don't feel so good and never knew why it helped so much. At first I thought I might have a food allergy, but I recently did a food allergy test and I actually have ZERO food allergies. Regarding your last point about the stages of grief, are you saying it's likely that I have celiac? I have Hashimoto's BTW and I know for sure that gluten causes an autoimmune response to my thyroid as my anti-TPO and anti-Tg go up and my throat feels swollen. Could the symptoms be due to that autoimmune response?
    • knitty kitty
      Hypothetical question about antibodies

      By knitty kitty · Posted

      @Zuma888, The antibodies produced in response to gluten are made in the intestines.  When the body is provoked sufficiently, the antibodies overflow out of the intestines and into the blood stream.  Once in the blood stream, the antibodies can be measured with tTg IgA tests.  Three grams of gluten per day for two weeks minimum is enough gluten to make you feel the symptoms of having been glutened, but the antibodies are not in sufficient quantity to be measured in the blood. Ten grams of gluten per day for a minimum of two weeks is required to get the anti gluten antibodies at a high enough level in the blood stream to be measured by tTg IgA tests. So, no, occasional cross contamination or (heaven forbid) intentional cheat days will not be sufficient for tTg IgA testing.  You will still be making antibodies which will still be causing inflammation and damage to the intestines and body.  Histamine is released as part of the immune response to gluten.  High histamine levels lead to food sensitivities, brain fog, and body aches.   The damage done to the gastrointestinal tract affects the absorption of essential vitamins and minerals.  Malabsorption of fats can cause changes in stools.  Insufficient absorption of vitamins and minerals can cause damage to other organs like the thyroid if it can't get enough Selenium, iodine, iron, zinc, and Thiamine.  Brain fog and fatigue can be caused by low Thiamine and other B Complex vitamins.  Vitamin D is needed to regulate the immune system.  One gene is all that's needed to develop Celiac disease.  I know a Celiac diagnosis is a change that can be difficult to get your head around.  Many people go through the five stages of grief.  One stage is "bargaining".  Sounds like you're stuck there.  Every little cheat counts to your detriment.  But sticking to a gluten free diet, makes every meal a success.   Read the comments below the article... Best wishes!  Keep us posted on your progress!
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